Figure 2

Design and implementation of the automated, microfluidic culture platform. (A) Illustration of the automated, microfluidic organoid culture platform, the Autoculture. (B) Front view images of the Autoculture. (1) Refrigerator with reagent reservoirs. (2) Syringe pump, distribution valves, and control interface. (3) Refrigerator with conditioned media collection reservoirs. These components reside on a lab bench directly above the cell culture incubator. (4) Microfluidic tubes enter through an incubator port and connect to the (5) microfluidic well plate chip inside the incubator. (6) Cross-sectional diagram of a single well containing an organoid culture.