Figure 4
Fabrication of the PDMS microfluidic chip. (A) Graphical rendering of the interlocking mold pattern for the PDMS substrate in the microfluidic chip assembly. (B) Interlocking mounts (blue, red, and green) affix to the base mold (purple) and define microfluidic geometries upon the poured PDMS that are retained as the substrate cures. (C) The PDMS substrate is removed from the mold and bonded to glass. (D) A cross-sectional rendering of the chip. Fluid enters from microfluidic inlets on the surface and follows channels sealed by glass on the bottom to wells with open access from the top. (E) A 3D-printed fluidic interface plate (yellow) connects 24 fluidic microtube lines with the inlets/outlets of the microfluidic chip. (F) Microfluidic chip (center) with an example of the fluidic interface plate (left) and fully installed fluidic interface plate (right).
