Figure 4
From: Novel method for detecting complement C3 deposition on Staphylococcus aureus
Variation in complement C3 deposition observed using a panel of genetically distinct MSSA and MRSA isolates. Each strain has been grown to mid-to-late exponential phase then normalised to OD600nm 2 before incubating with 2.5% normal human serum. C3 deposition was observed by incubating biotin-labelled Sbi-IV and streptavidin-AF488. Geometric mean fluorescence intensity (gMFI) measured using flow cytometry. Statistical differences were calculated using a one-way ANOVA with Dunnett posttest.
