Figure 3
From: PGC-1β maintains mitochondrial metabolism and restrains inflammatory gene expression
Effect of PGC-1β on metabolic phenotype of differentially-activated DCs.DCs were transduced with control shRNA or Ppargc1b shRNA and (a) ATP production rates by oxidative metabolism (JATPox) and glycolysis (JATPglyc) were calculated from Seahorse assay measurements (see Methods) at basal (left) and maximal (right) respiratory rates. (b) Total ATP production rates (JATPtotal) at basal metabolism and maximal respiration were determined by adding JATPox and JATPglyc from (a). ATP production rates by oxidative metabolism (JATPox) and glycolysis (JATPglyc) were calculated from Seahorse assay measurements in DCs stimulated with (c,d) LPS (100 ng/mL) or (e,f) IFN-β (1000 U/mL). Total ATP production rates of stimulated DCs in (d,f) at basal metabolism and maximal respiration were determined by adding JATPox and JATPglyc from (c,e). Colored circles are control DCs and open circles are DCs transduced with Ppargc1b shRNA. (g) AMPK phosphorylated at threonine 172 (p-AMPK T172), AMPKα, and β-actin, were visualized by western blot for DCs that were cultured in media containing 10, 1, or 0 mM glucose. Data are of (c,d) three or (a,b,e,f) five experiments pooled together, with each individual experiment represented by a circle and values from the same experiment joined by a line. Data in (g) are of one experiment representative of three experiments. Statistical significance was determined by paired t-test. *p < 0.05, **p < 0.01.
