Figure 5
From: Glycosphingolipid GM3 prevents albuminuria and podocytopathy induced by anti-nephrin antibody
Effect of anti-nephrin antibody and VPA on podocytes and interaction of nephrin and GM3. (A) Immunofluorescence staining images of nephrin (green) and GM3 (ref) in various treated podocytes. Yellow arrowheads showing merged areas of nephrin (green) and GM3 (red). (B) Immunofluorescence staining images of F-actin (red) in the same treated cells as A. Scale bars: 10 µm. Untreated (Untreat), anti-Nphs Ab treated (Ab treat), preVPA + anti-Nphs Ab treated (VPA + Ab treat) podocytes. (C) Immunoprecipitation (IP) using cell lysate of various treated podocytes and anti-nephrin and GM3 antibodies, and immunoblot (IB) analysis using anti-Nphs antibody. Cell lysate of untreated (Untreat), anti-Nphs Ab treated (Ab treat), preVPA + anti-Nphs Ab treated (VPA + Ab treat) podocytes.
