Figure 2
Imidazoquinoline UM-3001 demonstrated age-specific potency, effectiveness and IFNγ production in human newborn cord blood. (A–B) Human neonatal cord blood cultured in vitro for 6 h with buffer control (RPMI) or with increasing concentrations of various UM adjuvants. (C–D) Newborn versus adult blood cultured in vitro for 6 h with UM-3001. Supernatants were collected for ELISA. Results represent mean ± SEM, A-B; n = 7, C-D; n = 7. Cell supernatants were analyzed for cytokine expression by multiplex assay. Data are shown as fold change for newborn cord (blue line) over adult stimulated whole blood (black line) for 1 μM (E) and 10 μM (F) of UM-3001. (n = 6 adults, n = 8 newborns). For comparisons between overall groups (e.g., newborn vs. adult), two-way ANOVA followed by Tukey’s test for multiple comparisons was applied, and statistical significance denoted as *p < 0.033.
