Figure 6

Apoptosis markers in cancer cells after treatment with CuO NPs. (A) Apoptotic indicators for AMJ-13, MCF-7, and HBL-100 cells exposed to CuONPs at IC50 concentrations for 24 h and stained for 2 min using AO/EtBr. Unexposed cells have an intact structure. Nevertheless, CuONPs exposure correlated with apoptotic aspects indicated using red stains. Scale bar 10 µm. (B) Cells cycle phase. Sub-G1 phase concerning MCF-7 and AMJ-13 cells’ flow cytometry.