Figure 5

Observation and quantification of adhered MSCs by scanning electron microscopy (SEM). An MSC suspension was placed on the abraded porcine cartilage, and the cartilage was washed after a specified time and observed by SEM. (A) SEM images. (B) Quantification of adhered MSCs. The number of MSCs in a 320 µm × 380 µm area was counted in 5 or 9 fields per cylindrical cartilage piece. The number of cylindrical cartilage pieces per MSC donor was 2 or 3. This experiment was repeated 3 or 4 times independently and plotted (***p < 0.001).