Figure 7
From: Electron microscopy and calorimetry of proteins in supercooled water
Time-resolved ESEM of the condensation of water vapor, caused by a pressure excursion, on a myoglobin spot on copper. (a) t = 0 s and P = 126 Pa. No ice or SCW are detected. (b) t = 3 s and P = 135 Pa. SCW starts to appear on the protein spot and on the copper substrate. The red rectangle indicates a detail of SCW on the bare copper surface, and the yellow ellipse marks a copper zone free of SCW. (c) t = 6 s and P = 128 Pa. During the scan, SCW evaporates in the red zone. At the same time, ice growth is triggered inside the protein spot (see the dotted white line) and causes crystallization of neighboring SCW droplets by ice bridging. (d) t = 9 s and P = 129 Pa. Ice now covers the complete protein spot. It has also appeared in the yellow area, which is not in contact with the spot. SCW continues evaporating in the red zone. Modified with Power Point (www.microsoft.com/es-es/microsoft-365/powerpoint).
