Figure 2
Locomotion is impaired upon JAK/STAT inhibition in wild-type but not slbo-mutant border cells. (A–E) Single Z slices from movies show the delamination of wild-type (A), DN-Dome-expressing (B), slbo-mutant (C), and CytochalasinD-treated (D) border cells and a movie of posterior follicle cells (E). Red and blue circles indicate the cluster center and a border cell nucleus, respectively. (F) A schematic showing how cell movement was quantified by measuring the change in the angle of the border-cell nucleus (blue circle) relative to the cluster center (red circle). (G) The angular velocity of wild-type (WT), DN-Dome-expressing (Dome), slbo-mutant (slbo), and CytochalasinD-treated wild-type (WT + CytoD) and slbo-mutant (slbo + CytoD) border cells relative to the cluster center, and the angular velocity of posterior follicle cells relative to the posterior polar cell center (FC); 10 ≦ number of cells (n) ≦ 24, 5 ≦ number of egg chambers (N) ≦ 12. Error bars indicate SEM. ***p < 0.001. Time shown at the lower left indicates elapsed time from the start of the movie (white) and time prior to the delamination point (yellow). Numbers at the lower right indicate the oocyte length at the time point. For all images, anterior is left.
