Figure 1
From: The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotection
SMER28 protects primary neurons against excitotoxin-induced axon fragmentation. (a) Primary neuronal cells were stimulated with DMSO as vehicle control, 150 nM paclitaxel and 50 µM SMER28 in the presence or absence of 25 µM kainic acid for 6 h, as indicated. SMER28 treatment was applied 16 h before kainic acid was added. Cells were stained for β3-tubulin and visualized by spinning disk confocal microscopy. (b) Quantification of axon fragmentation. Axons were categorized according to examples shown above the graph as no fragmentation versus side axonal fragmentation or main axonal fragmentation. Data were collected form three independent experiments and represent means ± s.e.m; n = total number of axons analyzed. ****P < 0.0001, one way ANOVA.
