Figure 5
Proteomic Analysis of HUVECs stimulated with either PWO577 or SY007. HUVECs were stimulated for 16 h with either PWO577 or SY007 and then cell pellets were prepared. Protein extraction was then carried out on cell pellets followed by labelling, tryptic digest and analysis by LC–MS as described in “Materials and methods”. 5555 changes in protein expression, following PWO577 (upper left) or SY007 (upper right) treatment, are shown as volcano plots in the left-hand panels. Significant changes in protein expression (p > 0.05) are shown in light grey. Protein expression changes, associated with genes identified in Fig. 4 as being regulated by either PWO577 or SY007, are plotted in the right-hand panels. Significant protein expression changes (p > 0.05) are shown in light grey with changes in protein expression greater than 2-fold indicated either red (increase in gene expression) or green (decrease in gene expression). Non-significant protein changes are shown in black.
