Figure 6
Analysis of gene expression changes induced by IL-6 in the presence of PWO577 or SY007 in HUVECs. (A) To identify regulation of IL-6 gene expression by EPAC1 activators, confluent HUVECs were stimulated for the indicated times with IL-6 (5 ng/ml) plus sIL-6Rα (25 ng/ml) in the presence or absence of the indicated concentrations or D-007, PWO577 or SY007. Total RNA was then extracted and processed for RNAseq and plotted as a Venn diagram. (B) Gene expression changes from IL-6/sIL-6Rα-stimulated cells, compared to diluent treated control cells, were further analysed to identify common biological functions associated with gene expression changes by comparison with the Kyoto Encyclopaedia of Genes and Genomes (KEGG) database51,52. The occurrence of individual gene ontologies (Gene ratio) and their significance (p-adjust) are displayed as a dot plot. Arrows indicate the gene expression changes associated with functions associated with cytokine action, cancer and inflammatory pathways.
