Figure 1 | Scientific Reports

Figure 1

From: Nondestructive circadian profiling of starch content in fresh intact Arabidopsis leaf with two-photon fluorescence and second-harmonic generation imaging

Figure 1

Two photon fluorescence (TPF) and second harmonic generation (SHG) images of fresh intact wild-type Columbia-0 (Col-0) leaf and fresh intact starch excess 1 (sex1) mutant leaf. The last column shows the combined TPF and SHG images. The TPF images were acquired with a 670/30 nm filter, while SHG images were acquired with a 532/3 nm filter. The pixel dwell time was 0.016 ms and 0.128 ms for the TPF and SHG image acquisitions, respectively.

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