Figure 2

Spectral mapping for a fresh Col-0 leaf. (a) TPF (red) and SHG (green) imaging of a fresh intact wild-type Col-0 leaf. (b) Spectral mapping of the yellow box area in (a), with the total spectral intensity of the 655–685 nm band shown in red and the 530.5–533.5 nm band shown in green. (c) Selected spectra from the spectral mapping with 5 s integration, with the SHG peak feature visible.