Figure 4
From: Alloferon-1 ameliorates acute inflammatory responses in λ-carrageenan-induced paw edema in mice
Effect of alloferon-1 on the expression of inflammatory cytokines. (A) Heat map analysis of inflammatory cytokines in the serum detected by Luminex liquid cytokine chips (n = 3). The heatmap was generated by Microsoft Excel 2016 (Microsoft Corporation, Redmond, Washington). (B) Expression level of cytokines in serum. (C) Western blot analysis of IL-1β expression in mice in the serum (n = 3). The original blots are be supplemented in Original images of Fig. 4C. Prior to hybridization with the antibody during blotting, the blotting gel with 12 loading wells was cut into two equal parts. Thus, the full length of the blotting gel was 6 loading wells in total. (D) Quantification of the western blot signals with image J. Values are expressed as mean ± SEM. *P < 0.05 were considered as significant difference compared to the control group. Abbreviations: pg/ml, picograms/milliliter; IL, Interleukin; GM-CSF, Granulocyte- macrophage colony stimulating factor; TNF-α, Tumor necrosis factor-α; IFN-interferon-γ; MCP-1, monocyte chemoattractant protein-1; MIP-1, Macrophage inflammatory protein-1; G-CSF, Granulocyte colony stimulating factor.
