Figure 2 | Scientific Reports

Figure 2

From: Whole-embryonic identification of maternal microchimeric cell types in mouse using single-cell RNA sequencing

Figure 2

Gating conditions for sorting MMc cells. Noise and/or debris were removed with gate P1, and then doublet cells were removed with gate P2 and P3. Live-GFP positive cells (PerCP-Cy5-5 negative and FITC positive) were isolated by gate P4 and P5. (a), overall sorting result of a fetal cell sample. P5 gate was set for isolating MMc cells. Since all sorted cells cannot be plotted because of the counting limitation of processed cells even if the sample contains MMc cells, it will disappear from the display. (b) Therefore, we conducted index sorting at the same time. Panel (b) shows that 5 target cells were actually present and sorted. The images were created by default programs available in BD AriaIIIu (https://www.bdbiosciences.com/) and Adobe Photoshop (https://www.adobe.com, Photoshop 2021).

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