Figure 3

Transcriptomic analysis of brain endothelium in 1 year old APP/PS1 female mice and age-matched control female mice. (A) Volcano plot of DE mRNA transcripts analyzed in 1 year old APP/PS1 female mice against age-matched control female mice. The horizontal axis is the log2 fold change of transcripts. The vertical axis is statistically significant. Each dot represents an individual transcript (blue: down-regulated transcript; red: upregulated transcript; grey: no significant difference). The vast majority of differentially expressed genes are downregulated in the APP/PS1 female mice. (B) Gene ontology (GO) enrichment analysis of upregulated DE genes in the APP/PS1 female group versus control female group. (C) GO enrichment analysis of downregulated DE genes in the APP/PS1 female group versus control female group. Genes were considered upregulated in disease conditions if they were increased log2(fold change) > 1.00 with p < 0.05 and considered downregulated if log2(fold change) < − 1.00 with p < 0.05. The horizontal axis represents the number of genes for each GO term. (D, E) Heat map of the top 50 differentially expressed (upregulated and downregulated) endothelial genes between APP/PS1 females and control females. DEG (p < 0.05 and |log2 fold change| > 1), and their average expression values are presented in the side columns Each row represents one gene. Orange denotes higher gene expression and blue represents lower expression. Intensity of color is determined by a Z-score normalized by gene.