Figure 4 | Scientific Reports

Figure 4

From: Increased Type I interferon signaling and brain endothelial barrier dysfunction in an experimental model of Alzheimer’s disease

Figure 4

Validation of transcriptomic changes in the brain endothelium of APP/PS1 female mice. qPCR of RNA-Seq data based on log fold change and gene expression levels in brain EC of 1 year old APP/PS1 female and age-matched female control mice. Endothelial cells from the cortex of 1 year old APP/PS1 female mice demonstrated decreased Kif5c (A) and increased IFIT2 (C) gene expression supporting the RNA-Seq data. Data are represented as means and SD for three biological replicates and analyzed by Student’s T-test. **p = 0.0041; 0.0032 respectively. GAPDH is used as a housekeeping gene. (B, D) Representative confocal images of cytospin of brain EC from cortex of 1 year old APP/PS1 female and age-matched female controls further validated the qPCR results. Scale bar 20 µm.

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