Figure 1
Expression levels of anti-apoptotic Bcl-2 family members in NP and GSC-ECLs. (a) mRNA expression levels by RT-qPCR. rpl7 was used as normalizer. Graphs show mRNA expression fold change relative to NP. Bar charts represent the mean ± S.E.M. of three experiments. Student's t-test was used to detect significant differences between NP and each GSC-ECL. *P < 0.05, **P < 0.01, ***P < 0.001. a.u.: arbitrary units. (b) Protein levels were analyzed by Western blot. Actin was used as loading control. Representative blots of three independent experiments are presented. Full-length images are shown in Supplementary Figs. S11–S15. Bar graphs representing densitometric quantification of bands are depicted in Supplementary Fig. S1. (c) Transcriptional profile of anti-apoptotic Bcl-2 family members by RT-qPCR. rpl7 was used as normalizer. Bars represent the mean ± S.E.M. of three experiments. For each cell line, the Y-axis scale was set based on the range of the values in the corresponding data set.
