Figure 3

Involvement of Bcl-2, Bcl-w, and Bcl-xL in the control of GSC-ECL viability. (a) Percentage of cell death (PI⁺ cells) was determined by flow cytometry in G03 and G09 cells previously transfected with the indicated siRNA and treated or not with chemotherapeutic agents for 48 h. Non-targeting siRNA (NT siRNA) was used as a negative control. Each bar represents the mean ± S.E.M. of three independent experiments. Student's t-test was used to detect significant differences between NT-transfected cells and cells transfected with each specific siRNA. *P < 0.05, **P < 0.01, ***P < 0.001 (b) Percentage of cell death (PI⁺ cells) was determined by flow cytometry in G03 and G09 cells previously transfected with either NT, Bcl-2, Bcl-w, or Bcl-2 plus Bcl-w siRNAs, treated with WEHI-539 (1 μM) for 48 h and exposed or not to chemotherapeutic agents. Bar charts show the mean ± S.E.M. of three independent experiments. Student's t-test was used to detect significant differences between NT-transfected cells and cells transfected with each specific siRNA. *P < 0.05, **P < 0.01, ***P < 0.001. For each cell line, the Y-axis scale was set based on the range of the values in the corresponding data set.