Figure 8
The sensitizer role of Noxa depends on Mcl-1 activity. (a) Percentages of cell death (PI+ cells) were determined by flow cytometry in cells transfected with either NT or Noxa siRNA and exposed 48 h to WEHI-539 (1 μM) plus S63845 (0.1 μM). Bar charts show the mean ± S.E.M. of three independent experiments. Student's t-test detected no significant differences. (b) Percentages of cell death (PI+ cells) were determined by flow cytometry in cells transfected with Mcl-1 siRNA combined with either NT or Noxa siRNAs, and exposed 48 h to WEHI-539 (1 μM). Bar charts show the mean ± S.E.M. of three independent experiments. Student's t-test was conducted to detect significant differences between untreated and treated cells. *P < 0.05, **P < 0.01, ***P < 0.001. For each cell line, the Y-axis scale was set based on the range of the values in the corresponding data set.
