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Figure 1

From: Amiodarone improves anemia in a murine model of sickle cell disease and is associated with increased erythrocyte bis(monoacylglycerol) phosphate

Figure 1

Decreased expression of BMP is associated with increased sickling. (A) Heat map of significantly altered lipid species between washed erythrocytes drawn from SCD male mice, either PCSK9 deficient or replete (n = 3 per group, 16 weeks post-BMT). The p value of each lipid is listed to the right of the heat map. SM sphingomyelin, Cer ceramide, PC phophocholine, PE phosphatidylethanolamine. (B) Quantification of the percentage of sickled erythrocytes ± standard deviation after incubation with anti-IgG, anti-BMP antibody, Amiodarone (Amio, 1.5 μM), and/or Medroxyprogesterone acetate (Pro, 6.75 μM) for 4 h, with representative images (n = 4 mice, 3 fields of view each mouse). Significance was determined by an independent one-way ANOVA, followed by a post-hoc analysis with Turkey’s multiple comparisons tests (*p < 0.05, **p < 0.01).

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