Figure 1

ACSL1 is up-regulated in senescent K562 cells. (a–c) Apoptosis and the mean fluorescence intensity (MFI) of SPiDER-β Gal were measured by flow cytometry to determine the optimal concentration and treatment time of H₂O₂ in K562 cell line. (d,e) Cell senescence assay by SA-β-gal staining was applied to determine the optimal treatment time of imatinib plus Z-VAD in K562 cell line. (f,g) qRT-PCR was applied to quantify the relative expression of senescence-associated genes p21 and IL-6. (h,i) qRT-PCR and western blot analysis were applied to quantify the relative mRNA and protein expression of ACSL1. ns not significant, *p < 0.05, **p < 0.01 and ****p < 0.0001.