Figure 2
N-3 PUFA-enriched diet does not induce pulmonary inflammation. Analyses of lung and broncho-alveolar lavage fluid (BAL) from BALB/c mice that were fed a fish oil diet (FO) or an isocaloric control diet (CO) for 8 weeks. (a) Cytospin analysis of BAL cells. (b, c) Lung histology of CO (b) and FO (c) mice. Shown are representative lung sections stained with H&E, bar equals 250 μm. (d) Inflammatory score of histological sections. (e) Lung wet weights relative to body weight. (f–k) Expression of inflammatory markers in lung analyzed by Real-Time PCR: (f) IL-6. (g) IL-1β. (h) TNF-α (i) INOS. (j) Muc5AC; and (k) TRPM7. Data are means ± SEM of 5 animals per group. *p < 0.05. **p < 0.001, ***p < 0.0001. Shown are results of unpaired T-tests.
