Figure 7

Characterization and antibacterial activity of GO/CPC on human tooth substrate. (A) XPS elemental analysis. (B) Raman spectroscopy analyses. The red and blue lines indicate 1600 cm-1 and 1350 cm-1 (G and D bands), respectively. (C) Digital images of tooth substrate coated with GO/CPC. Only the lower half of the tooth substrate was immersed in 0.01% GO dispersion (top). No color change was observed of the GO/CPC coated area compared to UNT (bottom). (D) Fluorescent staining (top; green indicates live S. mutans) and quantification of the staining intensity of S. mutans cultured on each tooth substrate (bottom; n = 3, mean + standard deviation). *P < 0.05, **P < 0.01. Statistical analysis: two-tailed one-way ANOVA with post-hoc Tukey HSD test. CPC cetylpyridinium chloride, GO graphene oxide, UNT untreated.