Figure 5
From: Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy
LAMP3 causes salivary gland hypofunction through endolysosomal degradation of NKCC1 and AQP5 in vivo. Submandibular glands of C57BL/6 mice were instilled with AAV2-LAMP3 or AAV2-GFP (control), after which mice received weekly intraperitoneal injections of hydroxychloroquine (HCQ) at 60 mg/kg for 4 months. (A) Representative immunofluorescent images of submandibular glands showing staining for NKCC1 or AQP5 (both green) (scale bars = 20 µm). (B, C) Bar charts showing percentage of (B) NKCC1 and (C) AQP5 expressions per nuclear area in submandibular gland specimens. (D) Pilocarpine-stimulated salivary flow rate per body weight in 20 min. Values shown are mean ± SD (4 control, 5 LAMP3-overexpressing, and 4 HCQ-treated LAMP3-overexpressing mice). **P < 0.01 Student’s t-tests; Bonferroni correction used to correct for multiple testing).
