Figure 8
From: Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy
Graphical summary. LAMP3 overexpression decreases AQP5—a water channel in the apical membrane—and NKCC1—an ion cotransporter on the basolateral membrane—expressions in salivary gland epithelial cells by promoting endolysosomal degradation, resulting in salivary gland hypofunction. Simultaneously, LAMP3 increases internalization of AAV2 and enhances AAV2-mediated gene transduction efficiency via the activated endolysosomal pathway. AAV2-mediated AQP1 gene therapy induces ectopic AQP1 expression in both apical and basolateral membranes of salivary gland epithelial cells and creates a new pathway for fluid movement, leading to restoration of salivary flow rate in LAMP3-overexpressing mice.
