Figure 3

Flow cytometric determination of T cell, T helper cell subset, and macrophage proportions in the spleen. Proportions of CD4⁺CD8⁻ of CD3⁺ cells and CD4⁻CD8⁺ of CD3⁺ cells, and the ratio of CD4⁺CD8⁻/CD4⁻CD8⁺ cells. CD4⁺Foxp3⁺ cells, Treg cells (CD4⁺CD25⁺ Foxp3⁺), Foxp3⁺:RORγt⁺, CD11c⁺CD206⁻ of CD64⁺ cells, CD11c⁻CD206⁺ of CD64⁺ cells, M2 (CD64⁺CD11c⁻CD206⁺), and M1:M2 in spleen (n = 6 per group). Data obtained from each group were compared using one-way analysis of variance (ANOVA) followed by post hoc Tukey's multiple comparison tests. *Significant (P < 0.05) difference. CN chow diet and non-treatment control (saline, 200 μl/day), CP chow diet and methylprednisolone treatment (5 mg/kg/day), HN high fat-diet and non-treatment control (saline, 200 μl/day), HP high fat-diet and methylprednisolone treatment (5 mg/kg/day).