Figure 2 | Scientific Reports

Figure 2

From: Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization

Figure 2

Characterization of Tle1 phospholipase of T6SS-I of CH1476. (a) Growth curves of E. coli DH5α harboring pBAD33 derived constructions. (b) CFU counts at 8 h of culture of E. coli DH5α strains carrying pBAD33-derived constructions. (c) Toxicity assays performed on 0.2% arabinose-containing LB agar plates. Pictures are representative of three independent experiments. (d) SYBR/IP stained-cells ratio of E. coli DH5α strains carrying pBAD33-derived constructions. Data are expressed as means ± SEM (N = 9). P-values were derived from comparisons of each strain carrying pBAD33 with tle1 gene copy with cognate native pBAD33 plasmid or between tle1 containing pBAD33-ssOmpA and pBAD33-RBS strains via nonparametric Kruskal–Wallis and Dunn’s multiple comparison test: *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001.

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