Figure 2 | Scientific Reports

Figure 2

From: Combined transcriptome and metabolite profiling analyses provide insights into the chronic toxicity of carbaryl and acetamiprid to Apis mellifera larvae

Figure 2

Principal component analysis (PCA) and Partial least-squares-discriminant analyses (PLS-DA) score scatter plot of the metabolite profiles identified in bees exposed to acetamiprid (ACE), carbaryl (CR) and solvent control (SC) in POS (A, C) and NEG (B, D) ion modes. (A, B) Principal component (PC) 1 and PC 2 represent the first two principal components accounting for a total of 91.9% and 53.6% of the variance under the positive and negative ion mode conditions, respectively; (C, D) t[1] = first principal component. t[2] = second orthogonal component. In POS mode, the three significant components with R2X, R2Y and Q2 values was 0.544, 0.98 and 0.758, respectively. In NEG mode, the three significant components with R2X, R2Y and Q2 values was 0.346, 0.754 and 0.336, respectively. Red, green, and blue symbols represent ACE treated samples, carbaryl treated samplesa and solvent control, respectively. Each condition was analyzed by five biological replicates.

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