Figure 2
From: NT157 exerts antineoplastic activity by targeting JNK and AXL signaling in lung cancer cells
NT157 disrupts the cell cycle in lung cancer cells. (a) Cell cycle progression was determined by PI staining and flow cytometry in H1299 and H460 cells treated with vehicle or NT157 (6.4 and 12.5 μM). A representative histogram for each condition is illustrated. Bar graphs represent the mean ± SD of the percent of cells in subG1, G0/G1, S, G2/M, and > 4 N cells. Note that NT157 increased cell population at subG1 for both cell lines. In H1299 and H460 cells, NT157 increased cell population at S and G2/M phases, respectively. *p < 0.05, **p < 0.01 and ***p < 0.001; ANOVA and Bonferroni post-test. (b) Immunofluorescence analysis of H1299 and H460 cells treated with vehicle or NT157 (6.4 and 12.5 μM), displaying α-tubulin (green) and DAPI (blue) staining. The white arrows indicate pyknotic nuclei. Scale bar 100 µm.
