Figure 1
From: High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models
Identification of brain neuroanatomical areas in physiological/pathological conditions (mouse stroke models) by high-resolution micro-CT imaging (A) Overview of the technical steps required to prepare whole mice brains for micro-CT imaging. (B) Representative images of different brain projections (control, naive). Brains were stained with osmium tetroxide to reveal its neuroanatomical structures and differentiate white/grey matter. Regions such as cortex (Ctx), hippocampus and its sub-regions (HP, CA1-3, DG), choroid plexus (Cp), and striatum fibers in Caudate putamen (CPu) and thalamus (TH) are identified. (C) Representative 3D images of osmium staining to evaluate features induced by mouse stroke model (tMCAO) and TIA models, including brain edema (arrow indicates the brain swelling, comparison between contra/ipsilateral sides), total infarct area (dark area), core (highlighted darker area) and penumbra ((highlighted less dark area) discrimination, and striatum white fiber degeneration. Scale bar: 2 mm.
