Figure 2
From: High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models
Visualization of brain lesions in mouse stroke model (tMCAO) by high-resolution micro-CT imaging. (A) Virtual slices (~ 4 µm voxels) at different orientations from a micro-CT scan of a mouse stroke brain stained with osmium tetroxide, showing total lesion and core (orange/darker area) and penumbra ((yellow/grey area around the core) differentiation. The below panel shows lesion detail in the (B) transaxial and (C) sagittal planes. Scale bar: 2 mm. (D) Representative brain slices immunolabeled with specific neuronal markers (NeuN and MAP2), an apoptosis marker -TUNEL, confirming that the lesion identified in micro-CT images is similar to the histologically defined lesion. Scale bar: 500 µm. The right side panel shows the lesion in detail, in the coronal plane. Scale bar: 50 µm.
