Figure 4
Critical AD-risk modules and GABAergic subtypes among four species. (A) Heatmap (left) showed relative expression of 7 WGCNA modules among different cell types. Each module expression was calculated as an average expression of all genes in the corresponding module and scaled by columns. Heatmap (right) showed the number of overlaps between module genes and AD high-risk genes, and the significance (P value) of the hypergeometric distribution test identified the module in which AD risk genes were over-represented (P value: 0.01–0.05, *; 0.001–0.01, **). (B) Spearman correlation of snRNA-seq expression of AD susceptibility genes showing the conservation of each cell type genes associated with AD between human and each of three species on four cell types. (C) Venn plots showing DEG overlaps in each cell type between AD and control, AD risk genes, and turquoise module genes. (D) The above heatmap displayed the DEGs in four GABAergic subtype neurons, in which blue represented significantly DEGs (adjusted P value < 0.05). Expression patterns of genes (columns) were associated with AD by cell type (rows) in four species. The degree of disease-associated gene enrichment in cell type was calculated by the Wilcoxon Rank Sum Test. *P < 0.05.
