Figure 4 | Scientific Reports

Figure 4

From: Tandem duplication of a genomic region encoding glutathione S-transferase epsilon-2 and -4 genes in DDT-resistant Anopheles stephensi strain from India

Figure 4

Identification of duplication-breakpoints through inverted primerPCR (A) Genomic arrangement of GSTe genes in the susceptible mosquito (Che-S strain) as revealed through sequencing of L-PCR product amplified from primers E1F and E5R (shown with black harpoons). No amplification was found in Alw-R with these primers. (B) Identification of duplication breakpoints in resistant mosquitoes as revealed through sequencing of PCR product amplified from inverted primers E2R and E4F (shown with red harpoons). Inverted primer PCR amplification was successful only in the Alw-R mosquito. Part of the DNA sequence chromatograms displayed here is from the genomic region flanking duplication breakpoints in resistant mosquitoes and the corresponding region in the susceptible mosquito. The shaded part of the chromatogram is the insert DNA segment.

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