Table 3 Sequencing results for 11 Plasmodium genus positive samples based on the closest reference of Plasmodium species according to the BLAST search analysis of the SSU rRNA gene sequences. Samples from four cases (numbers 6, 12, 13 and 14 in Table 2) were not sequenced because the sequence length was < 100 bp. Samples from cases 7, 10 and 11 were sequenced using different primers or different Nest-1 products, or both.

From: A survey of simian Plasmodium infections in humans in West Kalimantan, Indonesia

No.

Primers

DNA sequence length (bp)

Closest Plasmodium species reference

Similarity

7

rPLU3 + rPLU4

183

P. inui clone B0362A91

100%

7

PlasmoM_N2F + PlasmoM_N2R

248

P. inui clone B0362A91

100%

8

rPLU3 + rPLU4

183

P. knowlesi isolate KM12k

100%

9

rPLU3 + rPLU4

183

P. knowlesi isolate KH96A-type

100%

10A

rPLU3 + rPLU4

174

P. knowlesi clone B0362B12

100%

10B

rPLU3 + rPLU4

174

P. fieldi clone LT3-B11

100%

11A

rPLU3 + rPLU4

183

P. inui clone B0362A91

100%

11B

rPLU3 + rPLU4

183

P. inui clone B0362A91

100%

11

PlasmoM_N2F + PlasmoM_N2R

226

P. knowlesi strain H

97.74%

15

rPLU3 + rPLU4

174

P. knowlesi clone B21-87

100%

16

rPLU3 + rPLU4

183

P. coatneyi isolate B136co

100%