Figure 6
Impact of transient inhibition of miR-223-3p expression by an antagomiR in CD80 and Arg1 as well as pro- and anti-inflammatory cytokines in lung tissue of GBS infected-mice. C57BL/6 male and female mice (n = 6/group) received intranasal injection of GBS alone (± 108 CFUs), or 50 µg/50 µL antagomiR-223, or 50 µg/50 µL scrambled RNA, 30 min before GBS infection and checked for changes in mRNAs of interest 6 h pi. The relative expression of miR-223-3p was evaluated in the lungs by real-time RT-qPCR. The values were normalized with two internal controls, snoRNA-202 and snoRNA-234. The relative expression of mRNAs CD80, Arg1, IL-1β, IL-10, CXCL2, CCL3, CCL2 and CXCL1 was evaluated in the lungs by real-time RT-qPCR. The values were normalized with two internal controls, RPL-19 and β-actin. The fold change values (2−ΔΔCt) are relative to naive mice matched by sex and age. Data are means ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001 (Mann–Whitney U test). Data are representative of two independent experiments.
