Figure 7

Sex difference in relative miR-223-3p expression in F4/80+ and Ly6G+ cells at 24 h post GBS lung infection. F4/80+ cells and Ly6G+ cells were purified from the lungs of GBS-infected or naive male and female mice (n = 6/group) using F4/80 and Ly6G immunomagnetic antibodies. Comparative analysis of miR-223-3p expression in the F4/80+ (A) and in the Ly6G+ (B) cell populations was evaluated by real-time qPCR. The values were normalized with two internal controls, snoRNA-202 and snoRNA-234. *p < 0.05 (Mann–Whitney U test). Data are representative of two independent experiments. The percentage of CD80+ and CD206+ macrophages were evaluated by flow cytometry with the gating strategy shown in Fig. S4. Correlation between the miR-223-3p expressed by the F4/80+ cells and the percentage of M1 or M2 macrophages (C). *p < 0.05, ****p < 0.0001 (Pearson χ² test).