Figure 4
PACE4-altCT splice variant silencing results in cell growth inhibition in JHU-LNCaP-SM cells. (A) PACE4 isoforms silencing determined by RT-qPCR with different concentrations of specific siRNA targeting PACE4-FL and (B) PACE4-altCT in JHU-LNCaP-SM cells using actin as housekeeping gene (n = 2 in duplicate). (C) Colony formation assay with representative wells of JHU-LNCaP-SM silenced for PACE4-FL and (D) PACE4-altCT at 5, 10 or 25 nM. Data are means ± SEM (n = 3). ****p < 0.0001 from a Student’s t-test.
