Figure 5
Expression of pro-GDF15 and GDF15 in LNCaP and JHU-LNCaP-SM cell lines. (A) RT-qPCR analysis of GDF-15 mRNA in LNCaP and JHU-LNCaP-SM cells using actin as a housekeeping gene, (B) GDF-15 concentration in conditioned media determined by ELISA assay (C) GDF15 and pro-GDF-15 levels in conditioned media and cell lysates respectively (two different gels). Two biological replicates are loaded side-by-side for each cell line. Data are means ± SEM. *p < 0.05 and ***p < 0.001 from a Student’s t-test.
