Figure 4

siRNA loading and releasing profile. (A) Loading profile of siRNA on [FA-PEGylated/PEI@GuIL@KIT-6] nanocarrier in 1 and 24 h incubation time at 4 and 25 °C. The best loading results of final complex achieved to 96% of loading in 24 h of incubation at 4 °C, with ratio of 1:35 of siRNA to [FA-PEGylated/PEI@GuIL@KIT-6] equivalent to 2.1 µM siRNA in combination with 48.3 µg of [FA-PEGylated/PEI@GuIL@KIT-6]. (B) Loading profile of siRNA on [FA-PEGylated/PEI@GuIL@KIT-6] nanocarrier with various pore sizes in 24 h incubation time at 4 °C (optimum condition). (C) The siRNA release from the [FA-PEGylated/PEI@GuIL@KIT-6] at 37 °C. Data are given as the means ± S.D. (n = 3).