Figure 6

In vitro biocompatibility, cytotoxicity and apoptosis inducing activity of [FA-PEGylated/PEI@ GuIL@KIT-6], siRNA@[FA-PEGylated/PEI@GuIL@KIT-6] and carboplatin. (A) and (B) MDA-MB-231 and MCF-10A cell viability after 24 and 48 h treatment with different concentrations of [FA-PEGylated/PEI@GuIL@KIT-6], respectively. (C) and (D) MDA-MB-231 cell viability under treatment of different concentrations of carboplatin and the cocktail of various concentrations of carboplatin plus consistent concentration of siRNA@[FA-PEGylated/PEI@GuIL@KIT-6] after 24 and 48 h, respectively. (The concentration of siRNA was consistent and equal to 2.1 µM). (E) Flow cytometry analysis of MDA-MB-231 cells treated with carboplatin and siRNA@[FA-PEGylated/PEI@GuIL@KIT-6]. Cells were stained with Annexin V-PE/7-AAD and quantitated by flow cytometry after 24 h of incubation. The cells treated (a) with PBS (negative control) (b) with IC₅₀ value of carboplatine and (c) cocktail of IC₅₀ value of carboplatin and siRNA@[FA-PEGylated/PEI@GuIL@KIT-6] equal to 2.1 µM siRNA. Data are given as the means ± SD. (n = 3).