Figure 8

In ovo antiviral efficacy of Thiazovivin against BPXV. (a) LD₅₀. Embryonated SPF chicken eggs, in triplicates, were inoculated with indicated concentration of Thiazovivin via CAM route. At 5 days post-Thiazovivin inoculation, eggs were visualized for viability of the embryos. LD₅₀ was determined by the Reed-Muench method. (b) Anti-BPXV efficacy (EC₅₀). Embryonated SPF chicken eggs, in triplicate, were inoculated with indicated concentration of Thiazovivin, followed by infection with BPXV at 100 EID₅₀. At 5 days post-Thiazovivin inoculation, eggs were visualized for viability of the embryos (c) Pock lesions on CAM at various drug regimens are shown. n = 3 independent experiments. Pair-wise statistical comparisons were performed using Student’s t test (***P < 0.001; *P < 0.05, NS nonsignificant).