Figure 1
Transcriptional changes in human aortic endothelial cells after exposure to intermittent hypoxia. Human Aortic Endothelial Cells (HAECs) were exposed to 60 cycles of IH (5%-20% O2) or normoxia (20% O2) (control). RNA-sequencing was performed from RNA isolated from 3 biological replicates. (a) Multidimensional scaling plot of differentially expressed genes in HAECs under IH compared to control normoxia. LogFC dim 1, and dim 2: base 2 logarithm of fold change dimension 1 and 2. (b) Volcano plot of -Log10 (p values) vs. Log2 fold change for DEGs and top significantly upregulated genes in IH. The -log10 (p values) represents the level of significance of each gene while log2 fold change represents the difference between the levels of expression for each gene. (c) Using qPCR, we measured mRNA expression of E-selectin (SELE), Fatty acid binding protein 4 (FABP4), Cytosolic phospholipase A2 gamma (PLA2G4C), and Hematopoietic progenitor cell antigen CD34 (CD34) in HAECs under control normoxia and IH (data are shown as mean ± SD, n = 6, biologic replicates). *p < 0.05. Parameters for DEG significance were set to absolute fold change ≥ 2 and FDR adjusted p value ≤ 0.05. Activated and suppressed (d) gene ontology biological processes (GO BP) and (e) activated KEGG pathways were identified by gene set enrichment analysis of DEGs in HAECs under IH compared to normoxia. The raw source data for RNA-seq are accessible via GEO (GSE205050). https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE205050.
