Figure 3
Epinephrine induces significantly higher number of DEGs compared to IH in human aortic endothelial cells. Differentially expressed gene profiles of HAECs, which were treated with epinephrine (10 µM) under normoxia or were exposed to 60 cycles of IH (5%-20% O2) perfusion system, were comparatively analyzed. (a) Heatmap of top significantly upregulated genes by IH in normoxia controls and IH- or epinephrine-treated HAECs. Z-score normalized expression values are shown in heat map, scale bar represents logFC values. “Pretty heatmaps R pheatmap package” from Z-score normalized expression values. https://cran.r-project.org/web/packages/pheatmap/pheatmap.pdf (b) Venn diagrams show differentially expressed genes (absolute fold change ≥ 2 and p value ≤ 0.05) in epinephrine-treated normoxic ECs (635 DEGs), and IH-exposed HAECs (184 DEGs). GO biological process (c) and KEGG pathway (d) enrichment analysis of gene set that were common between IH and epinephrine by Enrichr gene set search engine. GO biological process (e) and KEGG pathway (f) enrichment analysis of gene set that were specific to epinephrine by Enrichr gene set search engine.
