Figure 1
From: CdGAP maintains podocyte function and modulates focal adhesions in a Src kinase-dependent manner
CdGAP and β-PIX interact with Rac1 in podocytes. (A) Doxycycline-inducible myc-BirA (control, 37 kDa) or myc-BirA-Rac1G15A (60 kDa) were stably expressed in cultured human podocytes. Both cell lines were treated with doxycycline for 16 h as described in Experimental Procedures. Representative immunoblot for myc is shown. (B) After 16 h incubation with biotin and doxycycline, presence of biotinylated proteins was verified by immunoblotting for streptavidin. (C) Venn diagram of proximity interactions identified in BirA-Rac1G15A expressing podocytes and controls expressing BirA alone. (D) Heatmap representing normalized peptide counts toward each bait of three GEFs and two GAPs, which are significantly enriched in BirA-Rac1G15A expressing podocytes compared to controls.
