Figure 2

Butyrate treatment results in reduced ²⁵Mg²⁺ uptake in Caco-2 cells. (A) ²⁵Mg²⁺ uptake by Caco-2 cells after 20 min of treatment with increasing concentrations of Na-butyrate (2–8 mmol/L). Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). *P < 0.05 is considered statistically significant compared to control cells using One-Way ANOVA with Dunnett’s correction for multiple testing. (B) Time course of ²⁵Mg²⁺ uptake by Caco-2 cells treated with 8 mmol/L Na-butyrate (dashed line) or control (solid line) for 30 min. Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). *P < 0.05 is considered statistically significant compared to control cells at the same timepoint using Student’s t-test. (C, D) Fluorescence intensity of 2 µmol/L magnesium green in a 1 mmol/L MgCl₂ buffer with increasing concentrations of Na-butyrate (0–20 mmol/L) (C), and ATP (0–2 mmol/L) (D). Data represent mean ± SEM (n = 4 (C) and n = 3 (D), each experiment consisted of duplicates). (E) ²⁵Mg²⁺ uptake by Caco-2 cells after 20 min of treatment with 8 mmol/L Na-butyrate, or 8 mmol/L Na-butyrate and 1 mmol/L phloretin. Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). *P < 0.05 is considered statistically significant compared to control cells using One-Way ANOVA with Sidak’s correction for multiple testing.