Figure 4
Butyrate treatment affects AMPK signaling. (A–C) Representative immunoblot images of pAMPK (62 kDa) and total AMPK (62 kDa) of Caco-2 cell lysates after treatment with 8 mmol/L Na-butyrate, 25 mmol/L glucose, or 2 µmol/L oligomycin for 5 min (A), 10 min (B), and 20 min (C). (D–F) Densitometry analysis of pAMPK expression corrected for total AMPK expression levels after 5 min (D), 10 min (E), and 20 min (F) of treatment with 8 mmol/L Na-butyrate, 25 mmol/L glucose, or 2 µmol/L oligomycin. (G–I) Representative immunoblot images of pS6K (70 kDa) and total S6K (70 kDa) of Caco-2 cell lysates after treatment with 8 mmol/L Na-butyrate, 25 mmol/L glucose, or 2 µmol/L oligomycin for 5 min (G), 10 min (H), and 20 min (I). (J–L) Densitometry analysis of pS6K expression corrected for total S6K expression after 5 min (J), 10 min (K), and 20 min (L) of treatment with 8 mmol/L Na-butyrate, 25 mmol/L glucose, or 2 µmol/L oligomycin. Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). *P < 0.05 is considered statistically significant compared to control cells using One-Way ANOVA with Dunnett’s correction for multiple testing. All original immunoblots are presented in Supplementary Figs. S4–S5.
