Figure 5

Butyrate treatment reduces ²⁵Mg²⁺ uptake independent of ATP. (A–C) ²⁵Mg²⁺ uptake by Caco-2 cells after treatment with 8 mmol/L Na-butyrate and/or 2 mmol/L AICAR for 5 min (A), 10 min (B), and 20 min (C). Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). *P < 0.05 is considered statistically significant compared to control cells using One-Way ANOVA with Sidak’s correction for multiple testing. (D–E) ²⁵Mg²⁺ uptake by Caco-2 cells after treatment with 8 mmol/L Na-butyrate or 2 µmol/L oligomycin after 5 min (D). Time course of ²⁵Mg²⁺ uptake by Caco-2 cells after treatment with 8 mmol/L Na-butyrate or 2 µmol/L oligomycin at 5, 10, and 20 min (E). Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). *P < 0.05 is considered statistically significant compared to control cells at the same timepoint using One-Way ANOVA with Dunnett’s correction for multiple testing.